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KMID : 0383119530010040074
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Journal of Aerospace Medicine
1953 Volume.1 No. 4 p.74 ~ p.94
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Studies on Ferritin
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Abstract
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Granick, Hahn, Michaelis et al., american investigators, had taken an interest in biological significance of ferritin which was discovered by Laufberger in 1937, and they executed, in detail, basic researches on ferritin. Their researches on ferritin greatly contributed to our knowledge on iron metabolism. But all the above-mentioned research works are the studies in relation to the physicochemical properties of ferritin and the distribution of ferritin in normal animals and, the basic physilogical singinicance of ferritin But the systemic studies on, changes of ferritin in animals under normal or pathologic conditions are not yet pursued. Therefore the author has keenly felt the necessity of the systemic research on ferrittin metabolism, and executed some fundamental experiments on ferritin metabolism.
The main results obtained are as follows:
I. A new method for ferritin measurement using the blood-cell counting chamber.
There are several methods for ferritin measurement at present, but we have only one for measuring the ferritin content in small tissue fragments, namely Granick´s teased preparation (Slide glass method). This method is excellent for qualitative proof of ferritin, but has considerable disadvantages in quantitative measurement. Therefore the author has devised a method of using the blood-cell counting chamber for the measurement of ferritin content, and that is mentioned inn part I of this treatise.
II. Fundamental studies on ferritin metabolism.
1). In adult animals and human bodies the distribution of ferritin are different from one another. In rabbit the organs storing ferritin are spleen, testis and intestinal mucosa, in guinea pig and human bodies, liver, spleen and intestinal mucosa and the degree of ferritin content in each organ follows the same order, Therefore, the distribution of ferritin in rabbit differs from that of guinea pig and human bodies.
2). The distribution of ferritin in the animal undergoing experimental anemias differs according to the types of anemia. These phenomena are attributed to erythropoiesis and destruction of red cells.
3). In animals whose reticulo-endothelial system (R. E. S.) is blockaded, ferritin formation is unsatisfactory in spite of adequate iron supply, so that the author believes that R. E. S. has a close relation in the ferritin formation.
4). When the organ storing ferritin in normal condition is completely deprived of its function, other organs which are ontogentically or phylogeneticilly able to produce ferritin, form and store ferritin in compensation for the lost function.
5). During the fetal period the liver monopolizes the function of ferritin formation in rabbit, guinea pig and human bodies and the amount of ferritin content in the liver increases as the fetal period advances. Such characteristic distribution of ferritin in the fetal period gradually turns to that of adult animals during the period of lactation. In brief, the place of ferritin formation changes genetically.
6). In animals like rabbit whose ferritin content is comparatively small in nonpregnant condition, the amount of ferritin increases in pregnancy to meet the excessive demand of iron. But the animals like guinea pig whose ferritin content is large.
in non pregnant condition, there occurs no significant changes in ferritin metabolism in pregnancy.
7). It is observed that the ferritin content in rabbit testis shows little changes when undergoing the experimental anemias, the experiment on the relation of ferritin and R. E. S. and the experiment of compensatory ferritin formation and so it is believed that the biological significance of the ferritin in the rabbit testis differs from that of other organs of the rabbit.
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